TABLE 3.
Promoter activity using an R. sphaeroides cbbI::lacZ promoter fusion in R. capsulatus wild-type strain SB1003 and strain RCP under photoheterotrophic growth conditions
| Photoheterotrophic growth condition | β-Galactosidase activitya of strain:
|
|
|---|---|---|
| SB1003 | RCP | |
| Malate-ammonia | 15 ± 6 | 1,233 ± 166 |
| Malate-ammonia-DMSO | 13 ± 6 | 734 ± 263 |
| Malate-glutamate | 20 ± 5 | 422 ± 157 |
| Malate-glutamate-DMSO | 8 ± 2 | 364 ± 90 |
a
β-Galactosidase activities (mean ± standard deviation) are expressed as nanomoles per minute per milligram and were determined in three independent cultures of each strain (assayed in duplicate) containing plasmid pVKD1 from R. sphaeroides.