TABLE 3.
Oligonucleotide primers used for plasmid constructions
| Region | Template | Primer, sequencea |
|---|---|---|
| aadA | pWM5 | aadA-5′, GCAATCGATACGGATGAAGGCACGAACC; |
| aadA-3′, GCAGCGGCCGCTCGGCTTGAACGAATTGTTAG | ||
| dhfrIIb | p34E-Tp | dhfrIIb-5′, GCAGGCGCCACGAACCCAGTTGACATAAGC; |
| dhfrIIb-3′, GCAGCGGCCGCTTAGGCCACACGTTCAAGT | ||
| gen | pBBR1MCS-5 | gen-5′, GCAGGCGCCCACGAACCCAGTTGACATA; |
| gen-3′, GCAGCGGCCGCCTTGAACGAATTGTTAGG | ||
| ParaB | pBAD33 | ParaB-5′, AACTGCAGCGCCATTCAGAGAAGAAACCAA; |
| ParaB-3′, GCAGTCGACCATATGAATTCCTCCATCCAAAAAAACGGGTATGGAGA | ||
| PrhaS | pAH151 | PrhaS-5′, GCATCCGGAAATTCGCGACCTTCTCG; |
| PrhaS-3′, GCATCTAGAGCATATGGGCCTCCTGATGTCGTC | ||
| Psyn1-pstS∗ | pLD81 | Psyn1-5′, GCATCCGGAACTAGTGTCTTCAAGAATTCTAGG; |
| pstS-3′, CGGGATCCACGCGTTTACAAAGTC | ||
| xisλ | λ | xisλ-5′, CCGGAATTCTTGCGTGTAATTGCGGAGAC; |
| xisλ-3′, GGAAGATCTCCTTCGAAGGAAAGACCTGATGC | ||
| attPHK022 | pEY109 | attPHK022#1, GCAGCTAGCTAATGCTCTGTCTCAGGTC; |
| attPHK022#2, GCACCATGGGACAAAATTGAAATCG | ||
| intHK022 | pEY109 | intHK022-5′, GCACCATGGTAAGTAGGTCATTATTAGTC; |
| intHK022-3′, GCAGAATTCGTAGCCTTTTGAAGAGG | ||
| xis-intHK022 | pEY109 | xisHK022-5′, GCAGAATTCTGCGGAGACTTTGC; |
| intHK022-3′, see above | ||
| attPP21 | pBS1attP6-1 | attPP21#1, GCACCATGGAATGACCGACCGATA; |
| attPP21#2, GCAGCTAGCATAAGGCCTCGCAA | ||
| intP21 | pBS1attP6-1 | intP21-5′, GCAGAATTCAGAACCGCAACTCCCAA; |
| intP21-3′, GCACCATGGATAACGGGCGTATAACA | ||
| xis-intP21 | pBS1attP6-1 | xisP21-5′, GCAGAATTCGCAGCTAAGAGGAGGAC; |
| intP21-3′, see above | ||
| attPP22s | pJL110 | attPP22#1, GCAGCTAGCCGTTGTTACCGATCAAT; |
| attPP22#2, GCACCATGGAAGGCACGAATAATACG | ||
| attPP22 | P22 | attPP22#3, GCAGCTAGCCATTATGAAAATCAGCGGATTCGGA; |
| attPP22#4, CATGCCATGGAATCACCTGACTGAACATGCTCGAC | ||
| intP22 | P22 | intP22-5′, GCAGAATTCCACCACACGACAAGCCT; |
| intP22-3′, GCACCATGGACTCCTATTATCGGCAC | ||
| xis-intP22 | P22 | xisP22-5′, GCAGAATTCCTACGACATGCCTAACG; intP22-3′, see above |
| attPφ80s | pJL10 | attPφ80#1, AACCGGTGAATCACGAC; |
| attPφ80#2, GCACCATGGACATCCTTGAAAGCCTG | ||
| attPφ80 | pJL10 | attPφ80#3, GCATCTAGAGGTGAATCACGACAAAGCGTATC; |
| attPφ80#4, CATGCCATGGGCCCGTGCGAATCAGAAATAAT | ||
| intφ80 | pJL10 | intφ80-5′, GCAGAATTCGCTAGCAACCCTTATCCAGCA; |
| intφ80-3′, GCACCATGGCAATCAAAGAGTGGGAG | ||
| xisφ80 | pJL10 | xisφ80-5′, GCAGAATTCGGCAACTGGAGAGAGCTAT; |
| xisφ80-3′, GCAGCTAGCAAGAGATCATCGGAGAG |
a
Restriction sites are italicized. Bases complementary to the template are underlined.