TABLE 5.
PIX cloning efficiencies
attP Phage | CRIM plasmid | No. of Gmr or Kmr transductants per PFUa |
---|---|---|
λ | pAH63 | 2.3 × 10−5 |
HK022 | pAH70 | 1.6 × 10−5 |
φ80 | pAH153 | 1.9 × 10−4 |
P21 | pAH95 | 3.9 × 10−5 |
P22 | pAH154 | 1.8 × 10−7 |
Values are normalized to the I1v+ transducing titers. The same P1kc lysates yielded ca. 10−4 to 10−3 I1v+ transductants per PFU.