FIG. 2.

Chromosomal complementation of the N. meningitidis hemO deletion mutant with pigA gene from P. aeruginosa. (A) Southern blot analysis was performed on chromosomal DNA from the wild type and the mutant; hybridization and detection were achieved with either a hemO probe (panel 1) or a pigA probe (panel 2). (B) For each construct, complementation is shown by growth around a heme disk on GCB-Desferal media in the presence of IPTG.