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Absorption spectra of the heme-PigA(HO) complexes and SDS-PAGE gel of the purified PigA(HO) protein (inset). The spectra are ferric (—), ferrous deoxy (—), and ferrous CO bound (—) heme-PigA(HO). (Inset) SDS-PAGE gel of the purified PigA(HO). Lane 1, markers; lane 2, purified native PigA(HO).
