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HPLC chromatogram of the product of the heme-pigA(HO) reaction with ascorbate following extraction and methylation. (Inset) An HPLC chromatogram of a mixture of all four biliverdin dimethyl esters as standards is shown. The UV-visible spectra and the HPLC product analysis conclude that oxidative cleavage of heme by PigA(HO) is regiospecific with the biliverdin IX-β and that small amounts of biliverdin IX-δ isomers are formed.
