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. 2023 Mar 8;45(1):2187229. doi: 10.1080/0886022X.2023.2187229

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© 2023 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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graphic file with name IRNF_A_2187229_F0008a_B.jpg — (A) The mRNA expression of IL-17, RORγt, FoxP3 and IL-10 in kidney tissues 24 h after CLP operation. compared with the CLP + IgG group, *p < .05, ^#p > .05; compared with CLP + soluble-Tim-3 group, ^★p > .05. (B) The Western blotting expression and quantitative analysis of IL-17, RORγt, FoxP3 and IL-10 in kidney tissues. The protein levels are presented as mean ± SD. compared with the CLP + IgG group, *p < .05, ^#p > .05; compared with CLP + soluble-Tim-3 group, ^★p > .05. (C) The percentage of Th17 cells relative to CD4+ T cells were measured by flow cytometry after the preparation of single-cell suspensions. the values are mean ± SD. Comparisons between groups were performed using Student t-test. compared with the CLP + IgG group, *p < .05, ^#p > .05; compared with CLP + soluble-Tim-3 group, ^★p > .05. (D) The percentage of Treg cells relative to CD4+ T cells were measured by flow cytometry after the preparation of single-cell suspensions. the values are mean ± SD. Comparisons between groups were performed using Student t-test. compared with the CLP + IgG group, *p < .05, ^#p > .05; compared with CLP + soluble-Tim-3 group, ^★p > .05.

graphic file with name IRNF_A_2187229_F0008b_C.jpg — (A) The mRNA expression of IL-17, RORγt, FoxP3 and IL-10 in kidney tissues 24 h after CLP operation. compared with the CLP + IgG group, *p < .05, ^#p > .05; compared with CLP + soluble-Tim-3 group, ^★p > .05. (B) The Western blotting expression and quantitative analysis of IL-17, RORγt, FoxP3 and IL-10 in kidney tissues. The protein levels are presented as mean ± SD. compared with the CLP + IgG group, *p < .05, ^#p > .05; compared with CLP + soluble-Tim-3 group, ^★p > .05. (C) The percentage of Th17 cells relative to CD4+ T cells were measured by flow cytometry after the preparation of single-cell suspensions. the values are mean ± SD. Comparisons between groups were performed using Student t-test. compared with the CLP + IgG group, *p < .05, ^#p > .05; compared with CLP + soluble-Tim-3 group, ^★p > .05. (D) The percentage of Treg cells relative to CD4+ T cells were measured by flow cytometry after the preparation of single-cell suspensions. the values are mean ± SD. Comparisons between groups were performed using Student t-test. compared with the CLP + IgG group, *p < .05, ^#p > .05; compared with CLP + soluble-Tim-3 group, ^★p > .05.