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. 2022 Nov 9;8(2):e10448. doi: 10.1002/btm2.10448

FIGURE 5.

FIGURE 5

The hscNT promotes cell survival and neuronal maturation in the lesion center. (A and B) hscNPCs differentiate into neurons expressing the immature neuron markers Tuj‐1 (a) and DCX (b) at 7 days. (c) Bar chart showing the percentage of Tuj‐1+/GFP+ and DCX+/GFP+ cells among all GFP+ cells in the two groups at 7 days. (d) Bar chart showing the numbers of Tuj‐1+/GFP+ and DCX+/GFP+ cells in the two groups at 7 days (n = 6 images). (e,g) Immunofluorescence staining images showing Tuj‐1+ immature neurons (e) and NF+ (f) and MAP2+ (g) mature neurons in the injury center at 2 months. (h) Quantification of the percentage of Tuj‐1+/GFP+, NF+/GFP+ and MAP2+/GFP+ cells among all GFP+ cells in the SCI + L + P and SCI + L + P + A groups at 2 months (n = 10 images). (i) Quantification of the numbers of grafted GFP+ cells in the lesion center at 7 days (n = 6 images) and 2 months (n = 10 images). Error bars represent standard error, and two‐group comparisons were analyzed using Student's t‐test. NS indicates no significant difference. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.