Fig. 5.

Absence of in vivo selection of PD-1 edited CAR-T cells. (A) NGS analysis of infused T cell products and transferred CD45.1⁺ flow cytometry-sorted T cells, belonging to the experiment of Fig. 3 A–E. (B) Longitudinal blood analysis of PD-1-expression in CAR- and PD-1 KO CAR-T cells by flow cytometry. (C) Representative flow cytometry plots showing PD-1 frequencies of EGFRt⁺ transferred cells in the peripheral blood at the indicated time points after T cell injection.