FIGURE 4.

Polymorphic peptides from XaFliC, but not XcFliC, protected citrus from Xanthomonas citri (Xc) infection. (a) Natal leaves were infiltrated with a water suspension of Xc (OD₆₀₀ = 0.05) in the presence of the XcFliC (Xc) or XaFliC (Xa) peptides at a final concentration of 70 μM (areas surrounded by dashed lines). Canker symptoms were recorded 10 days after bacterial inoculation. Only peptides flgIII‐20 and flgIII‐27 inhibited canker formation. Details of the infiltrated leaf sectors at higher magnification (10×) are shown on the left and right. (b) Same experiment performed with Troyer citrange showing that only peptides flgIII‐20 and flgIII‐27 protected leaves from Xc infection. (c) Peptides flgIII‐20 and flgIII‐27 partially protected Mexican lime leaves from Xc infection. (d) Peptides flgIII‐20 and flgIII‐27 attenuated canker development caused by Xanthomonas aurantifolii (Xa) in Mexican lime leaves. Three leaves of each citrus variety were infiltrated and the images presented are representative of the phenotype observed in most infiltrated leaves