Skip to main content
. 2023 Jan 24;24(4):331–345. doi: 10.1111/mpp.13300

FIGURE 6.

FIGURE 6

XaFliC‐dependent expression of FLS2, WRKY22, and other defence‐related genes in sweet orange. Reverse transcription‐quantitative PCR analysis of Natal leaves challenged with Xanthomonas citri (Xc), XcΔfliC, XcΔfliC complemented with XaFliC, or water as control, 24 h after bacterial inoculation. (a) Relative expression levels of RLK genes showing XaFliC‐dependent expression of Xanthomonas aurantifolii (Xa)‐induced genes, including FLS2. (b) Relative expression levels of transcription factor‐encoding genes showing that the WRKY22 and MYB102 homologues are the most highly up‐regulated in response to XaFliC expression. (c) Relative expression levels of oxidative burst‐associated genes showing significant induction of the putative peroxidase gene PRX52 in leaves infiltrated with XcΔfliC complemented with XaFliC relative to leaves infiltrated with Xc or XcΔfliC. (d) Relative expression levels of hypersensitive response (HR)‐ and senescence‐related genes showing XaFliC‐dependent expression of the SAG13, ACD2, CYP3A, and CRR38 homologues. Error bars represent standard deviations of three independent biological samples, whereas asterisks denote statistically significant differences between the means at the α = 0.05 level relative to XcΔfliC (*) or Xc (**)