FIGURE 8.

Amino acid polymorphisms in flgIII‐20 and flgIII‐27 are found in several Xanthomonas species and pathovars. Protein sequence alignment of Xanthomonas FliC proteins showing that the amino acid polymorphisms found in flgIII‐20 and flgIII‐27 between Xanthomonas aurantifolii (Xa) and X. citri (Xc) are also present in several species/pathovars of Xanthomonas. The alignment, performed with ClustalW, includes the FliC regions spanning the flgIII‐20 and flgIII‐27 peptides from the following Xanthomonas species/pathovars: Xa (WP_007962409), X. citri pv. glycines WP_016848727, X. campestris pv. mirabilis WP_218495416, X. euvesicatoria WP_218195708, X. euvesicatoria pv. allii MCP3041826, X. citri pv. thirumalacharii WP_223574663, X. campestris pv. paulliniae WP_218557524, X. arboricola WP_055854457, X. perforans MCF5971967, X. dyei WP_104616676, X. prunicola WP_101364107, X. oryzae WP_134953848, X. oryzae pv. oryzicola AEQ96507, X. fragariae WP_254223128, X. hortorum WP_115038939, X. pisi WP_046964182, X. floridensis WP_064510802, X. cucurbitae WP_104603719, X. cannabis WP_047696158.1, X. bromi WP_065467843, X. vesicatoria WP_005994319, X. campestris WP_218559892, X. vasicola WP_010373629, X. axonopodis pv. passiflorae MBV6815034, X. axonopodis pv. manihotis WP_017154654, X. citri pv. malvacearum WP_003482972.1, and Xc (WP_003482972). A K‐to‐D change at position 6 and an S/T‐to‐N change at position 10 in flgIII‐20 distinguish a large group of Xanthomonas species/pathovars, including Xa, from the citri, passiflorae, manihotis, and malvacearum group. The nonconserved changes from S to V at position 6 and from S/G/A to T at position 9 in flgIII‐27 also distinguish the Xa‐related group from the Xc‐related group. Two serine residues at positions 10 and 17 in flgIII‐27 are replaced by N/D and A, respectively, between the Xa‐ and Xc‐related groups, except in X. fragariae, which has an asparagine residue at position 10