FIGURE 5.

PLE‐IL‐12‐NPs show an equivalent immune response to carrier‐free IL‐12. (a) Experimental design for immune profiling. Mice were inoculated with orthotopic HM‐1 tumors and dosed with 10 μg IL‐12 IP in PLE‐IL‐12‐NPs, UL‐NPs, or carrier‐free and compared to vehicle control for 3 daily doses 14 days after inoculation. (b,c) Immune population statistics found within the tumor environment as measured by flow cytometry. (d,e) Exhaustion indicators found in the tumor environment. (f,g) Memory response found in the tumor and spleen by flow cytometry. (h,i) Effector responses as measured by flow cytometry. Statistical differences were measured by the Student's t test. *p < 0.05; **p < 0.01; ***p < 0.001. N = 6 (Dextrose, ULNP, LNP), N = 5 (Free IL‐12) unless reduced due to insufficient events during analysis