Fig. 2.
Reduced FDX1 releases ES-bound Cu in vitro. (A) A schematic representation of in vitro assay to determine FDX1-mediated release of Cu from ES. (B) UV/Vis spectra of 50 µM human FDX1Red /FDX1Ox ± 50 µM ES–Cu(II) in Tris buffer (10 mM Tris, 50 mM NaCl, pH 7.5). (C) UV/Vis spectra of the BCS–Cu(I) complex in the presence of increasing concentrations (0 to 55 µM) of FDX1Red or (D) FDX1Ox protein. FDX1Ox/Red proteins were mixed with 1 mM BCS in Tris buffer, and 20 µM ES–Cu(II) was added to start the reaction. The final dimethyl sulfoxide (DMSO) concentration in the solution was 20% after injection.