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. 2023 Feb 27;120(10):e2120536120. doi: 10.1073/pnas.2120536120

Fig. 3.

Fig. 3.

Retraction fiber-based mitotic dynamics. (A, i) Illustration showing convention for metaphase plate orientation (α) (scale bar, 20 µm), (ii) analysis of individual temporal profiles of metaphase plate orientation using orientation change per interval and maximum range of metaphase plate oscillations, (iii and iv) statistical comparison across all fiber configurations and 2D for metaphase plate dynamics, and (v) mitosis duration analysis showing 1F-elongated to be the slowest. (B) Metaphase plate orientation and maximum range of oscillations decrease with increasing RFC. (C) Mitosis duration decreases with increasing fiber coverage. (D) Representative image showing the mechanical linking of the astral microtubules and the CRTX at the sites of the RFs, actin (magenta), microtubules (cyan), histone H2B (olive), and kinetochore Hec1 (red). (Scale bars, 5 µm and 1 µm in the main image and Inset, respectively.) Model formulations based on increased effective friction and force for MP movements at higher retraction fiber coverage. *, **, ***, and **** represent P < 0.05, 0.01, 0.001, and 0.0001, respectively.