Fig. 5.

Division axis orientation and daughter cell positioning on suspended fibers. (A) Polar histograms of cell division axis orientations (marked by a red arrow; division axis orientation in fiber networks is measured with respect to the undeflected horizontal fibers; for 2D-rounded category, the division axis orientation is measured with respect to the long axis of the interphase cell shape) shown for different fiber geometries; absolute acute angle magnitudes of orientation are used. n = 45, 41, 62, 105, and 33 for 1F-elongated, 2F-elongated, MF-elongated, 2F-kite, and 2D-rounded, respectively. Percentage of cells in 0 to 15° sector for aligned geometries and 30 to 60° for crosshatch geometry. (Scale bar, 20 µm.) (B) Time-lapse images showing the representative dynamics of cell division axis (red dashed line) in single fibers versus multiple fibers. (Scale bar, 20 µm.) (C, i) Representative profiles showing the variations of division axis orientation and subsequent positioning of daughter cells. (ii and iii) Statistical comparison of the orientation change per interval and maximum range of division axis movements for different substrate categories. *, **, ***, and **** represent P < 0.05, 0.01, 0.001, and 0.0001, respectively.