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. 2023 Mar 2;19(3):e1011055. doi: 10.1371/journal.ppat.1011055

Fig 6. Binding of C4BP prevents internalization of CEACAM-binding Gc by neutrophils.

Fig 6

(A) The indicated variants of Gc were incubated with C4BP (purple), or PBS (grey). The percentage of neutrophils with≥ 1 phagocytosed bacterium was calculated as in Fig 5C. Data represent the mean ± SEM of ≥3 independent experiments. Statistical analyses were performed by two-way ANOVA followed by Sidak’s multiple comparisons. (B) Opaless Gc was incubated with C4BP (50 ug/mL), opsonized in rabbit anti-Gc IgG (80 μg/mL, 20 minutes, 37°C), sequentially incubated with IgG then C4BP, or left untreated. Unopsonized Gc and IgG-opsonized Opaless Gc bound similar amounts of C4BP (S8E Fig). The percentage of neutrophils with ≥ 1 phagocytosed bacterium was calculated as in (A). Statistical analyses were performed by one-way ANOVA followed by Tukey’s multiple comparisons. Opaless Gc incubated with C4BP behaved similarly to Opaless Gc (not shown). *p<0.05, **p<0.01.