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. 2023 Mar 9;6:0068. doi: 10.34133/research.0068

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Copyright © 2023 Jinhong Cai et al.

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Fig. 8. — Histological evaluation of OA therapy. (A) H&E staining images of OA joints after therapy. (B) Safranine O-fast green staining images of OA joints after therapy and the corresponding Mankin scoring at 4 and 8 weeks (C). Immunohistochemical staining results of OA joints after treatment: IL6 (D) and MMP13 (E). The corresponding groups were as follows: normal rats (Sham), OA rats by PBS treatment (OA), OA rats by MH treatment (MH), OA rats by Cu_1.5MH treatment (Cu_1.5MH), and OA rats by Cu₆MH treatment (Cu₆MH). (“*” symbol compared with OA group, *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001, and “^#” symbol compared between groups, ^#P < 0.05 and ^##P < 0.01).