FIG. 2.
Fluorescence microscopy of live B. subtilis cells grown at 25°C. (A) Exponentially growing cells expressing CspB-GFP (MW2). (B) MW2 cells treated with chloramphenicol for 30 min. (C) MW2 cells 3 h after entry into the stationary phase. (D) MW2 cells treated with rifampin for 30 min. (E) Cells expressing HBsu-GFP 3 h after entry into the stationary phase. The white lines indicate septa between cells. Bars = 2 μm. Note that immunofluorescence using anti-CspB antibodies with fixed wild-type cells confirmed the delocalization of CspB upon inhibition of transcription (D) (data not shown).