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. 2001 Nov;183(21):6435–6443. doi: 10.1128/JB.183.21.6435-6443.2001

FIG. 3.

FIG. 3

Fluorescence microscopy of live B. subtilis cells growing in rich medium. DAPI, DNA stain; Nomarski, outline of cells; membrane stain, vital stain FM4-64. (A) Wild-type cells. (B) Two fields of 64BD cells (cspB::spc cspD::cat) grown at 37°C. The arrow indicates an abnormal nucleoid structure. (C) Strain MW3 (cspB-gfp cspC::kan) growing at 25°C. The arrow indicates a minicell devoid of DNA. (D) Cells depleted of HBsu (see text) for 3 h. The arrow indicates an anucleate cell. The white line indicates a septum dissecting a nucleoid. (E) Wild-type cells 3 h after the onset of sporulation at 37°C. The arrows indicate polar septa. (F) 64BD cells (cspB::spc cspD::cat) 3 h after the onset of sporulation. (G) Wild-type cells 2 h after the onset of sporulation. The arrows indicate condensed nucleoids in forespore compartments that have proceeded to stage III (engulfment) of sporulation. The white lines indicate septa. (H) Cells of strain 64BD (cspB::spc cspD::cat) 2 h after the onset of sporulation. The white lines indicate septa. Note that nucleoids are more decondensed and extended in cells entering sporulation (G and H) than in growing cells (A and B).