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. 2022 Nov 23;22(3):e13741. doi: 10.1111/acel.13741

FIGURE 1.

FIGURE 1

Neuronal HLH‐30/TFEB is a regulator of longevity. (a) (Left panel) Presence and (Right panel) absence of HLH‐30::GFP intestinal misexpression (arrowhead, GFP‐enriched intestinal nucleus) under combinatorial regulation from the rab‐3 promoter (rab‐3p) and unc‐54 or rab‐3 3′ untranslated region (3′UTR), respectively, as detected with RNAi knockdown of the nuclear exportin gene xpo1, (b) Lifespan analyses of wildtype, hlh‐30(tm1978), and neuronal HLH‐30/TFEB rescued, hlh‐30(tm1978) animals fed OP50 at 25°C. (c, d) Lifespan analyses of daf2(e1370), daf‐2(e1370);hlh‐30(tm1978), and neuronal HLH‐30/TFEB rescued daf2(e1370);hlh‐30(tm1978) animals fed (c) OP50 and (d) control RNAi (L4440) or RNAi against daf‐16 at 25°C. Animals were developed on OP50 at 20°C and shifted to 25°C on (a to c) OP50 or (d) bacteria expressing RNAi from day 1 of adulthood. Data are representatives of 3 independent replicates and comparisons were made by Mantel‐Cox log‐rank. See Figures S1 and S2, Tables S1–S3 for additional details on lifespan analyses.