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. 2023 Mar 15;133(6):e158357. doi: 10.1172/JCI158357

Figure 5. B cell proliferation measured by CFSE dilution.

Figure 5

(A) Gating strategy for proliferation of CD3-CD19+ B cells. (B) Proliferation of unstimulated compared with TE stimulated B cells in patients with AGS (left, n = 28) and healthy controls (right, n = 15), Wilcoxon matched-pairs signed rank test, **P < 0.01 and ***P < 0.001. (C) Comparison of patients with AGS and individuals acting as healthy controls, Mann-Whitney U test, ***P < 0.001, n = 28 (AGS) and n = 15 (controls). (D) Effect of removing α-Gal from the TE in patients with AGS (left, n = 10) and individuals acting as healthy controls (right, n = 13), Wilcoxon matched-pairs signed rank test, *P < 0.05. (E) B cell proliferation in response to different doses of TE in patients with AGS, Friedman test with Dunn’s multiple comparisons test, **P < 0.01, ***P < 0.001, n = 22. (F) Effect of inhibition with anti-CD40L and anti-IL-4 antibodies in patients with AGS, Friedman test with Dunn’s multiple comparisons test, **P < 0.01, n = 16. (G) Comparison of B cell proliferation in unstimulated cells and cells stimulated with an α-Gal containing nontick protein in patients with AGS (left, n = 20) and individuals acting as healthy controls (right, n = 10), Wilcoxon matched-pairs signed rank test, *P < 0.05. (H) Comparison of proliferation in naive (CD27-IgD+) and memory (CD27+) B cells in patients with AGS, Wilcoxon matched-pairs signed rank test, n = 28). Each point within the box plot represents 1 individual. Box plots represent IQR and median, whiskers extend to the farthest data points.