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. 2023 Mar 15;133(6):e161944. doi: 10.1172/JCI161944

Figure 7. 1-TbAd reduces macrophage control of M. tuberculosis.

Figure 7

(A–C) Mouse BMDMs were infected with M. tuberculosis (MOI = 2) for approximately 6 hours, pulsed with TbAd for 3 hours, and then treated with ATRA (10 μM), CH223191 (3 μM), or DMSO. Macrophages were infected with M. tuberculosis, with or without a 20 μM TbAd pulse, followed by measurement of CFU (A) bacterial luminescence reporters (B and C) for 10 days (C) or 7 days (B). (D) Macrophages were infected with WT M. tuberculosis, pulsed with TbAd, and treated with ATRA (10 μM) for 6 days prior to CFU measurement. Statistical comparisons in A–C)were performed using an ordinary 1-way ANOVA with Tukey’s or Šídák’s multiple-comparison test (all comparisons tested, P values are shown where P < 0.05). Comparisons with multiple time points (A and B) were performed on AUC data. Statistical comparison of slopes in D was performed using an unpaired t test.