Figure 7. YAP/TAZ mediates STRN4-PP2Ac regulation of STING signaling in macrophage.

(A) GSEA of YAP targeted genes in PP2Ac^KO versus PP2Ac^WT BMDM treated with cGAMP using RNA-Seq data set from Figure 1A. (B) PP2Ac^KO and PP2Ac^WT BMDMs were treated with cGAMP and protein expression was analyzed. (C) CTL, PP2Ac^KO and YAP overexpressed PP2Ac^KO THP-1 differentiated macrophages were treated with cGAMP and protein expression was analyzed. (D) shYAP THP-1 differentiated macrophages were treated with cGAMP for 4 hours and ISGs expression was measured. (E) YAPs94A overexpressed THP-1 differentiated macrophages were treated with cGAMP for 4 hours. ISGs expression was measured (F) YAPs94A overexpressed THP-1 differentiated macrophages were treated with cGAMP and protein expression was analyzed. (G) Publicly available RNA-Seq data set of sorted MDM and MG from human glioma samples, and blood MDM and nontumor MG were obtained. GSEA of YAP WT- and YAPS94A-targeted genes in GBM MDM versus blood MDM and GBM MG versus nontumor MG. Data are from 1 experiment representative of 3 independent experiments. Error bars depict SEM. P values were calculated by 1-way ANOVA with Tukey’s multiple comparison test. *P < 0.05, ****P < 0.0001.