Figure 8. Tumor-induced YAP expression to suppress STING signaling in macrophage.

(A) Scheme of experimental workflow. (B) Histological analysis of CTL and PP2A^KO THP-1 differentiated macrophages stained for YAP (green) and nucleus (DAPI, blue). Scale bar: 10 μm. Image representative of 4 independent regions (n = 4). (C) Quantification of YAP fluorescent intensity in selected regions. (D) YAP expression in TCM treated CTL and PP2A^KO THP-1 differentiated macrophages. (E and F) After 5 days in ACM or TCM, CTL or PP2Ac^KO THP-1–differentiated macrophages were treated with cGAMP for 4 hours. CXCL10 expression was measured. (G) After 5 days in ACM or TCM, CTL or STRN4^KO THP-1–differentiated macrophages were treated with cGAMP for 1.5 hours. MST1/2 antibody was used for co-IP and blotted for PP2Ac and MST1/2. (H) Model of PP2Ac/STRN4 mediated STING-IFN suppression in TAMs. cGAMP given at 10 μg/mL. Data are from 1 experiment representative of 3 independent experiments. Error bars depict SEM. P values were calculated by 1-way ANOVA with Tukey’s multiple comparison test. ****P < 0.0001.