Figure 3.

Characterization of HMD-G fusion to base editors in HEK293T cells

(A) Comparison of the C-to-T base-editing efficiencies of BE4max and eeBE4max at 24 endogenous targets in HEK293T cells. (B) Comparison of the A-to-G base-editing efficiencies of ABEmax and eeABEmax at 15 endogenous targets in HEK293T cells. (C) Summary of the cumulative C-to-T base-editing efficiencies of multiple BE4max variants and multiple eeBE4max variants at endogenous targets for “NGG” PAM and “non-NGG” PAM in HEK293T cells. (D) Summary of the cumulative A-to-G base-editing efficiencies of multiple ABEmax variants and multiple eeABEmax variants at endogenous targets for “NGG” PAM and “non-NGG” PAM in HEK293T cells. (E) Frequency of indels by BE4max and eeBE4max. Each point represents the average indel frequency at a target site. (F) Frequency of indels by ABEmax and eeABEmax. Each point represents the average indel frequency at a target site. Data in (A) and (B) represent means ± SD (n = 3 independent experiments). The statistical analysis of (C), (D), (E), and (F) was performed by using paired two-tailed Student’s t test. ∗∗p < 0.01, ∗∗∗p < 0.001; ns, not significant.