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. 2023 Feb 24;26(3):106267. doi: 10.1016/j.isci.2023.106267

Figure 6.

Figure 6

Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway and Gene Ontology (GO) enrichment analysis of differentially expressed genes (DEGs) as determined by RNA-seq

(A) The top 30 DEGs between ALS-PDC-affected and-unaffected cerebral organoids.

(B) and among ALS-PDC-unaffected, ALS-PDC-affected, and ALS-PDC-unaffected/BMAA-treated cerebral organoids.

(C) Significantly downregulated KEGG pathways between ALS-PDC-affected and-unaffected organoids.

(D) GO term enrichment analysis showing the downregulated biological processes between ALS-PDC-affected and-unaffected organoids.

(E and F) The expression of selected genes was validated by RT-qPCR. The data were analyzed using the Student’s t test in GraphPad Prism 8. All values were compared to the ALS-PDC-unaffected condition and expressed as means ± SEM. ∗p< 0.05, ∗∗p< 0.01. ∗∗∗p< 0.001,∗∗∗∗p< 0.0001.