REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Rabbit polyclonal anti- microtubule associated protein 2 (Map2)	Santa Cruz Biotechnology	Cat #sc20172; RRID:AB_2250101
Mouse monoclonal anti- ionized calcium binding adapter molecule 1 (Iba1)	Abcam	Cat # ab15690; RRID:AB_2224403
Mouse monoclonal anti-interferon inducible transmembrane protein (IFITM)	Santa Cruz Biotechnology	Cat # sc-374026; RRID:AB_10116884
Rabbit monoclonal anti CD86	Abcam	Cat # ab239075; RRID:AB_2921417
Mouse monoclonal anti- mannose receptor (CD208)	Abcam	Cat # ab8918; RRID:AB_306860
Chemicals, peptides, and recombinant proteins
Recombinant human interlukin-34 (IL34)	BioLegend	Cat # 577906
Recombinant human colonystimulating factor (CSF)	BioLegend	Cat # 574804
Recombinant human transforming growth factor beta 1(TGF-β)	Life Technologies Inc	Cat # PIRP8600
SB431542	Tocris Bioscience Inc.	Cat # 1614/1
Dorsomorphin	Tocris Bioscience Inc.	Cat # 3093/10
Deposited data: accession code PRJNA75348
ALS-PDC unaffected organoid 1 raw and analyzed data	This paper	SRR15404813
ALS-PDC unaffected organoid 2 raw and analyzed data	This paper	SRR15409003
ALS-PDC affected organoid 1 raw and analyzed data	This paper	SRR15409909
ALS-PDC affected organoid 2 raw and analyzed data	This paper	SRR15410515
Oligonucleotides
Primers for PCR	This paper	Supplemental table