Figure 4.

Frequency of AAV integrations

(A) Frequency of target reads with AAV integrations (AAV-SpCas9 + AAV-gRNA[s]) after dual-gRNA4+5 (n = 2, dark blue and red closed circles, 4,750× and 2,205× coverage) and single gRNA5 (n = 2, light blue and orange closed circles, 2,900× and 2,550× coverage) treatments in Pcp2-127Q and dual-gRNA4+5 (n = 2, dark blue and red open circles, 5,300× and 2,630× coverage) and single gRNA5 (n = 2, light blue and orange open circles, 4,400× and 3,450×) treatments in BAC-72Q. Each point represents a single mouse. Percent indels from Figures 2I–2L are shown below. (B) Frequency of target reads with AAV-SpCas9 (squares) or AAV-gRNA(s) (triangles) integrations at gRNA4 (n = 2, 5,200× and 2,460× coverage) and gRNA5 (n = 2, 4,300× and 1,950× coverage) sites in dual-gRNA4+5-treated Pcp2-127Q mice (closed shapes) and at gRNA4 (n = 2, 5,500× and 2,710× coverage) and gRNA5 (n = 2, 5,100× and 2,550× coverage) sites in dual-gRNA4+5-treated BAC-72Q mice (open shapes); sum of the AAV-SpCas9 mean and AAV-gRNA(s) mean integration frequency is denoted. Percent indels from Figures 2I–2L are shown below. (C) Frequency of target reads with AAV-SpCas9 and AAV-gRNA(s) integrations between gRNA4 and gRNA5 sites in dual-gRNA4+5-treated Pcp2-127Q mice (n = 2, 4,750× and 2,205× coverage) and BAC-72Q mice (n = 2, 5,300× and 2,625× coverage). (D) Frequency of target reads with AAV-SpCas9 and AAV-gRNA(s) integrations at the gRNA5 site in single gRNA5 treated Pcp2-127Q mice (n = 2, 2,900× and 2,550× coverage) and BAC-72Q mice (n = 2, 4,400× and 3,450× coverage); the sum of the AAV-SpCas9 mean and AAV-gRNA(s) mean integration frequency is denoted. Percent indels from Figures 2I–2L are shown below.