Figure 1.

Peripheral blood Vγ9Vδ2 T cell frequencies and phenotypes

(A) Representative flow cytometry gating strategy to identify CD3⁺ Vγ9Vδ2 T cells in cryopreserved PTM samples.

(B) Representative FACS plot and frequencies of Vδ2⁺ T cells, Vγ9⁺Vδ2⁺ T cells, and Vγ9⁻Vδ2⁺ T cells, as a percentage of the total CD3⁺ T cell population in peripheral blood mononuclear cells from adult male pigtail macaques (Macaca nemestrina). The FACS plot shows Vγ9⁺Vδ2⁺ T cells (magenta), Vγ9⁻Vδ2⁺ T cells (cyan), and total CD3⁺ T cells (black).

(C) Representative FACS plot and frequencies of naive (T_N; CD28⁺CD95⁻), central memory (T_CM; CD28⁺CD95⁺), and effector memory (T_EM; CD28⁻CD95⁺) T cells. Plot shows Vγ9⁺Vδ2⁺ T cells (magenta) and Vγ9⁻Vδ2^- T cells (black).

(D) Representative FACS plots illustrating CD26 expression with CD94 (human) or NKG2A (pigtail macaque) expression, on peripheral blood Vγ9Vδ2 T cells.

(E) Frequencies of CD26/NKG2A expression on peripheral blood Vγ9Vδ2 T cells in pigtail macaques.

(F) CCR5, CCR6, CXCR3, IL-18R, and DNAM-1 expression on peripheral blood Vγ9Vδ2 T cells relative to CD26 and NKG2A co-expression.

Each point on the graphs represents an individual animal for each blood sample. Lines and error bars indicate median and interquartile range. All analysis was performed on cryopreserved PBMC samples (n = 8–12 animals from 6 to 7 independent experiments). Statistics assessed by two-tailed Wilcoxon test (B and C), Friedman test with Dunn’s multiple comparisons correction (E), or Kruskal-Wallis test with Dunn’s multiple comparisons correction (F). ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.