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. 2023 Feb 25;26(3):106269. doi: 10.1016/j.isci.2023.106269

Figure 4.

Figure 4

Phenotype of in-vivo-expanded Vγ9Vδ2 T cells in the blood

Phenotyping was performed on freshly isolated peripheral blood mononuclear cells (n = 7 animals; 1–2 per treatment group).

(A) Representative FACS plot and frequencies of central memory (TCM; CD28+CD95+) and effector memory (TEM; CD28−CD95+) Vγ9Vδ2 T cells pre- and post-antigen administration. Expanded Vγ9Vδ2 T cells (Day 4) are plotted in magenta, while pre-expanded Vγ9Vδ2 T cells (Day 0) are plotted in cyan. Vγ9−Vδ2- T cells are plotted in black.

(B) Representative histograms and frequencies of CD69 expression on Vγ9Vδ2 T cells pre- and post-antigen administration.

(C) Representative histograms and frequencies of CCR5, CCR6, and CXCR3 on Vγ9Vδ2 T cells pre- and post-antigen administration.

(D) Representative histograms and median fluorescence intensities (MFI) of granzyme B (GzmB) expression in Vγ9Vδ2 T cells pre- and post-antigen administration.

Each point on the graphs represents an individual animal from each timepoint. Data collected from 1 experiment. Statistics assessed by Friedman test with Dunn’s multiple comparisons correction across all 4 groups (A). ∗p < 0.05.