Figure 5.

In vitro expansion of CCR6-sorted Vδ2⁺ T cells

CCR6⁺ and CCR6^- CD3⁺Vδ2⁺ T cells were sort purified from cryopreserved pigtail macaque splenocytes, and expanded for 13 days in autologous, Vδ2-depleted PBMCs with Zol (15 μM) and rhIL-2 (1x10³ IU/mL) (n = 5 animals from 3 independent experiments).

(A) Schematic of in vitro CCR6-sorted Vδ2⁺ T cell expansion with Vδ2-depleted PBMCs. Diagram created with BioRenderer.com.

(B) Representative histogram of sorted CCR6⁺ (magenta) and CCR6^- (cyan) Vδ2⁺ T cells. Vδ2^- T cells are plotted in black. Sort gating strategy presented in Figure S3.

(C) FACS plots illustrating MACS-based Vδ2⁺ T cell depletion from autologous PBMCs.

(D) Fold change in expanded CCR6-sorted Vγ9Vδ2 T cells from baseline levels, calculated as described in the method details.

(E) Granzyme B (GzmB), CCR6, and CXCR3 expression on in-vitro-expanded, CCR6-sorted Vγ9Vδ2 T cells or whole PBMCs.

Each point on the graphs represents an individual animal for each condition. Lines and error bars indicate median and interquartile range. Statistics assessed by two-tailed Wilcoxon test (D) or Friedman test with Dunn’s multiple comparisons correction (E). ∗p < 0.05.