Figure 6.
Phosphoantigen and rhIL-2 driven phenotypic changes in pigtail macaque Vγ9Vδ2 T cells
Cryopreserved PBMCs (n = 8 from 4 independent experiments) were expanded in vitro for 13 days with Zol (15 μM) and rhIL-2 (0.1x103, 0.5x103, or 1.0x103 IU/mL).
(A) Representative FACS plots illustrating the shift in CCR6 and granzyme B (GzmB) expression in in-vitro-expanded Vδ2+ T cells with increasing concentrations of rhIL-2, relative to baseline frequencies.
(B) CCR6 (%) and GzmB (MFI) expression frequencies in in-vitro-expanded Vδ2+ T cells with increasing concentrations of rhIL-2, relative to baseline frequencies.
(C and D) Representative histograms and frequencies of CCR6 (C) and GzmB (D) expression on splenic-derived Vγ9Vδ2 T cells, following 72 h in vitro stimulation as described in the method details (n = 4–7 per group from 3 independent experiments). Additional data presented in Figures S4 and S5.
Each point on the graphs represents an individual animal for each condition. Lines and error bars indicate median and interquartile range. Statistic assessed by Kruskal-Wallis test with Dunn’s multiple comparisons correction (B–D). ∗p < 0.05, ∗∗p < 0.01.