FIG. 4.

Proteins differentially expressed with (pink) or without (green) 50 mM acetic acid. (A) Strain W3110 ackA-pta was grown in LBK broth–50 mM MOPS–50 mM TES, pH 6.7, to an OD₆₀₀ of 0.4. (B) Strain W3110 was grown in glycerol M63 salts–50 mM MOPS–50 mM TES, pH 6.7, to an OD₆₀₀ of 0.2. Other growth and gel conditions were as for Fig. 2.