| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Experimental models: Organisms/strains | ||
| NCLXfl/fl mice | Luongo et al.,48 | N/A |
| B6.Cg-Tg(Camk2a-cre)T291-Stl/J | The Jackson Laboratory, USA | Stock # 005359 |
| NCLXfl/fl x Camk2a-Cre | This Study | N/A |
| Antibodies | ||
| MCU (1:1000) | Sigma | Cat # HPA016480 |
| MCUb (1:1000) | Abgent | Cat # AP12355b |
| MICU1 (1:500) | Tomar et al.,101 | Custom generation by Yenzyme |
| MICU2 (1:500) | Abcam | Cat # ab101465 |
| MICU3 (1:500) | Sigma Aldrich | Cat # HPA024771 |
| EMRE (1:1000) | Santa Cruz Biotechnology Inc. | Cat # sc-86337 |
| NCLX (1:500) | Santa Cruz Biotechnology Inc. | Cat # sc-161921 |
| VDAC (1:2500) | Abcam | Cat # ab15895 |
| OxPhos Cocktail | Abcam | Cat # MS604 |
| Total APP (22C11, 1:1500) | Chemicon International | Cat # MAB348 |
| BACE1 (1:500) | Sigma | Cat # MAB5308 |
| ADAM10 (1:500) | Chemicon International | Cat # AB19026 |
| PS1 (1:500) | Sigma | Cat #S182 |
| Nicastrin (1:200) | Cell Signaling | Cat # 5665 |
| APH-1 (1:200) | Millipore | Cat # AB9214 |
| HT7 (1:200) | Thermo Fisher Scientific | Cat # MN1000 |
| AT180 (1:200) | Thermo Fisher Scientific | Cat # MN1040 |
| AT8 (1:200) | Thermo Fisher Scientific | Cat # MN1020 |
| AT270 (1:200) | Thermo Fisher Scientific | Cat # MN1050 |
| PHF13 (1:200) | Cell Signaling | Cat # 9632 |
| 4-HNE | Abcam | Cat # ab48506 |
| SYP (1:500) | Santa Cruz Biotechnology | Cat # sc-55507 |
| IBA-1 (1: 250) | Proteintech | Cat # 10904-1-AP |
| PSD-95 (1:250) | Invitrogen | Cat # MA1-045 |
| GFAP | Proteintech | Cat # 60190-1-Ig |
| Beta-Tubulin (1: 1000) | Abcam | Cat # ab6046 |
| Anti-Mouse (1:10000) | Licor | Cat # 925–68070 |
| Anti-Goat (1:10000) | Licor | Cat # 926-32214 |
| Anti-Rabbit (1:10,000) | Licor | Cat # 926–32211 |
| Oligonucleotides | ||
| Rps13-F | GCACCTTGAGAGGAACAGAA | N/A |
| Rps13-R | GAGCACCCGCTTAGTCTTATAG | N/A |
| Nclx-F | GCCATCTCCACTAACCTCAAA | N/A |
| Nclx-R | GGGTCTGAGAAAGCCACTAAA | N/A |
| Recombinant DNA | ||
| gRNA #1 | GGGCCTCTACGTGTTCTACGGTTTTAGAG CTAGAAATAGCAAGTTAAAATAAGGCTAG TCCGTTATCAACTTGAAAAAGTGGCACCG AGTCGGTGC |
N/A |
| gRNA #2 | CTCACCTGGTGTCTCCGATAGTTTTAGAG CTAGAAATAGCAAGTTAAAATAAGGCTAG TCCGTTATCAACTTGAAAAAGTGGCACCG AGTCGGTGC |
N/A |
| Chemicals, peptides, and recombinant proteins | ||
| Paraformaldehyde | Sigma | Cat #P6148 |
| Vector Elite ABC HRP Kit | Vector Laboratory | Cat # PK-6100 |
| DAB Substrate Kit | BD Biosciences | Cat # 550880 |
| RIPA buffer | EMD-Millipore | Cat # 20-188 |
| SIGMAFAST™ Protease Inhibitor Cocktail | Sigma-Aldrich | Cat #S8830 |
| Phosphatase inhibitor | Sigma-Aldrich | Cat # 04906837001 |
| Formic acid | Sigma | Cat # 33015 |
| Human Aβ (1-40) ELISA Kit | Wako Chemicals USA | Cat # 298-64601 |
| Human Aβ (1-42) ELISA Kit | Wako Chemicals USA | Cat # 298-62401 |
| Protein Assay Dye Reagent | Bio-Rad | Cat # 22660 |
| PVDF Immobilon-FL membrane | EMD Millipore | Cat # IPFL00010 |
| Blocking buffer | Rockland | Cat # MB-070 |
| Dihydroethidium (DHE) | Thermo Fisher Scientific | Cat #D11347 |
| KCl | Sigma-Aldrich | Cat #P9333 |
| Cresyl Violet | Sigma-Aldrich | Cat# C5042 |
| Glacial acetic acid | Sigma-Aldrich | Cat# A6283 |
| KH2PO4 | Sigma-Aldrich | Cat #P5655 |
| MgCl2 | Sigma-Aldrich | Cat # 449172 |
| NaCl | Sigma-Aldrich | Cat # 746398 |
| DAPI | Thermo Fisher Scientific | Cat #P36981 |
| Software and algorithms | ||
| Fiji ImageJ software | Fiji ImageJ | https://imagej.net/software/fiji/ |
| Zen 2010 | Carl Zeiss | https://www.zeiss.com/microscopy/en/products/software/zeiss-zen.html |
| GraphPad Prism 9 | GraphPad Software | https://www.graphpad.com/scientific-software/prism/ |
| ImageJ Pro plus software | Meyer Instruments | https://www.meyerinst.com/mediacybernetics/image-pro-plus/ |
| PACKWIN software | Harvard Apparatus | https://www.harvardapparatus.com/packwin-software-panlab.html |
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