Fig. 4.

Adipocytes from the visceral depot are more diet-responsive than adipocytes from the subcutaneous depot. A, Venn diagram showing differentially regulated proteins in response to WD in isolated adipocytes from the two depots. B, distribution of log₂ fold changes in response to WD in visceral and subcutaneous adipocytes (VAdi and SAdi, respectively). C, proteins abundances after WD across all proteins in VAdi (x-axis) and SAdi (y-axis) with correlation coefficient for direction and magnitude of changes. D, Z-scored protein abundances of selected proteins within pathways that change in both depots in response to WD. E, changes in all mitochondrial proteins detected in VAdi or SAdi after WD. F, abundances of proteins in complexes I-VI of the electron transport chain in VAdi after WD relative to SAdi. G–H, label-free quantitation for BCL-2-associated X (BAX), BCL-2-related ovarian killer protein (BOK), apoptosis regulator Bcl-2 (BCL2), Myeloid leukemia cell differentiation protein Mcl-1 homolog (MCL1), peroxisome proliferator-activated receptor gamma (PPARG), BCL2/adenovirus E1B 19 kDa protein-interacting protein 3 (BNIP3) and E3 ubiquitin-protein ligase MARCHF5 (MARCHF5). I, Z-scored protein abundances of selected proteins within pathways uniquely changing in VAdi in response to WD. J, protein differences in smooth-muscle proteins in VAdi and SAdi in mice fed either chow or WD. K, Difference in protein abundance of typical smooth muscle proteins in adipocytes from different depots within same diet. C, Chow; W, WD, Western diet.