Fig. 4. Effects of emestrin J (10) on cell apoptosis and cell-cycle arrest in 786-O cells. Emestrin J (10) induced apoptosis of 786-O cells (a, b) and arrested the cell cycle (c, d) in the G2/M phase. 786-O cells were treated with vehicle (DMSO, Cont) or 10 (2 and 4 μmol/L) for 24 h, as indicated. All data shown above are mean±SD of three independent experiments. ^** P<0.01, ^*** P<0.001 vs. Cont. DMSO: dimethylsulfoxide; Cont: control; SD: standard deviation; FITC: fluorescein isothiocyanate; PI: propidium iodide; PI-A: PI-area.