Table 1.
Primers for qRT-PCR assay.
| Genes | Accession number | Primer sequence (forward; reverse) | Amplicon (bp) | Concentration (nM) |
|---|---|---|---|---|
| HMBS | XM_417846 | GGTTGAGATGCTCCGTGAGTTT; GGCTCTTCTCCCCAATCTTAGAA |
153 | 300 |
| RPL1 | NM_205322.1 | TCTCCACGACGACGAAGTCA; CCGCCGCCTTGATGAG |
63 | 600 |
| TBP | NM_205103 | CTTCGTGCCCGAAATGCT; GCGCAGTAGTACGTGGTTCTCTT |
82 | 300 |
| CLDN1 | NM_001013611.2 | AGACCCAGGAGTATGGCGGC; AGACCCAGGAGTATGGCGGC |
170 | 600 |
| CLDN2 | NM_001277622.1 | GACGTGAACCATTCGCAGTCC; GTTTTGTGAGGGCACAGGCA |
151 | 300 |
| TJP1 | XM_015278981.2 | TGAAGGACCCCAGTGACACG; CACTCATGGCTGGGAGCGTA |
75 | 600 |
| ZO2 | NM_204918.1 | GCCCCAGCCAAAAGCAGTTA; CCAGCAAGTCGCAGACCAAC |
126 | 600 |
| OCLN | NM_205128.1 | CGCTACGGCAAAGCCAACAT; CCATCCGCCACGTTCTTCAC |
92 | 600 |
| LGR5 | XM_046909876.1 | ACGTCTTGCAGGAAATGGCT; TTGGCATCCAGGCGTAGAG |
159 | 300 |
| SOX9 | NM_204281.1 | CCCCACCATGTCGGATGACT; CCTTCTTCAGGTCCGGGTCG |
119 | 600 |
| CHGA | XM_015287755.2 | AAGCCAACACTGATGAAGAAGG; CTGAGGTGAGTACTGGGAGC |
70 | 300 |
| MUC2 | XM_421035.2 | ATTGAAGCCAGCAATGGTGT; TTGTTGGCCTTGTCATCAAA |
125 | 300 |
| LYZ | NM_205281 | GACGATGTGAGCTGGCAG; GGATGTTGCACAGGTTCC |
225 | 600 |
| IL1B | NM_204524.1 | TGCCTGCAGAAGAAGCCTCG; AGGTCGCTGTCAGCAAAGTCC |
170 | 600 |
| TNFA |
NM_204267.1 |
CCCTACCCTGTCCCACAACC; TGGGCGGTCATAGAACAGCA |
150 | 300 |
| IFNG | NM_205149.1 | ATGTAGCTGACGGTGGACCT; GCGGCTTTGACTTGTCAGTGT |
135 | 300 |
| IL10 | NM_001004414.2 | CACCGCTTCTTCACCTGCGA; CCCGTTCTCATCCATCTTCTCG |
81 | 800 |
| CNR1 | XM_015284635.2 | TTCCAAAGACGTCTGCACCCC; GTTGGAGCCCACGTAAAGGA |
185 | 300 |
| FAAH | XM_422450.5 | TCCCTCGGTGGACATGGTCT; TGGGTCTCGCTTTCCTGC |
174 | 300 |
| NAPEPLD | NM_001030730.3 | GAAGAGGACGGGACTGCTCG; GTCACTGCCCCTGGATCCTC |
153 | 300 |