Table 3.
Primer sequences for real-time PCR amplification.
| Genes | GenBank ID | Primer sequences | Product length (bp) |
|---|---|---|---|
| ZnT1 | XM_421021.5 | F: 5′-AGAGCCTGGGTTTGGATTCG-3′ | 229 |
| R: 5′-AGCCCATGCATGAACACTGA-3′ | |||
| ZnT4 | XM_423325 | F: 5′-GCCATCTTGACGGACGTAGT-3′ | 190 |
| R: 5′-CAAGGTACACCAGGACACCC-3′ | |||
| ZnT5 | NM_001031419.2 | F: 5′-ATGGAGGAAAAGTACAGCAGCC-3′ | 118 |
| R: 5′-TCAGAAACTTGGCGAAGCAC-3′ | |||
| ZnT7 | NM_001008788.1 | F: 5′-TGCTGCCCCTCTCCATTAAG-3′ | 114 |
| R: 5′-AGAGGTTGCGGGATGTCTTG-3′ | |||
| ZnT9 | XM_015285471.1 | F: 5′-ACATGTTTTTCCGTGCAGCC-3′ | 265 |
| R: 5′-CGGAACACAACCTTTACCAGC-3′ | |||
| ZnT10 | XM_015283897.1 | F: 5′-GAGCTGTAGAGATGGGTCGC-3′ | 184 |
| R: 5′-ACACCGAGCAAACCGATGAT-3′ | |||
| ZIP3 | XM_015299966.2 | F: 5′-CATACATCCAGGAGGCAGAGG-3′ | 246 |
| R: 5′-CCTGGATGATCTTGACGGGG-3′ | |||
| ZIP5 | XM_025145573.1 | F: 5′-CCAAGATGAAACGCACGCAA-3′ | 284 |
| R: 5′-TAAGCTGCGACCAAGTCCTG-3′ | |||
| B0AT1 | XM_419056.6 | F: 5′-CTTGGGTGAGGTAGGTGGGA -3′ | 167 |
| R: 5′-GATGCGGGTGCTCTCATGTA-3′ | |||
| LAT1 | NM_001030579.2 | F: 5′-GGAAAGGCCCATCAAGGTGA-3′ | 248 |
| R: 5′-ACGATTCTTGGGGAACCAC-3′ | |||
| y+LAT2 | XM_001231336.4 | F: 5′-ATCTTGCGCCTGAGGGAAGG-3′ | 296 |
| R: 5′-CGGCTCTGAACTCCAATCTGT-3′ | |||
| rBAT | XM_004935370.3 | F: 5′-CCTAGGAGGAGAGGCACGAA-3′ | 223 |
| R: 5′-TCCTGCATAGGGCTGCAATG-3′ | |||
| EAAT3 | XM_424930.6 | F: 5′-CGTCCAGGCCTGTTTTCAAC-3′ | 171 |
| R: 5′-TCGGAATACATGCCCACGAT-3′ | |||
| PepT1 | NM_204365.1 | F: 5′-AAAACAGGTTTCGGCATCGC-3′ | 167 |
| R: 5′-CTGCTGGTCAAAAAGTGCCC-3′ | |||
| β-actin | NM_205518.1 | F: 5′-CGGTACCAATTACTGGTGTTAGATG-3′ | 163 |
| R: 5′-GCCTTCATTCACATCTATCACTGG-3′ | |||
| GAPDH | NM_204305.1 | F: 5′-CTTTGGCATTGTGGAGGGTC-3′ | 128 |
| R: 5′-ACGCTGGGATGATGTTCTGG-3′ |
ZnT1, zinc transporter 1; ZnT4, zinc transporter 4; ZnT5, zinc transporter 5; ZnT7, zinc transporter 7; ZnT9, zinc transporter 9; ZIP3, Zrt-irt-like protein 3; ZIP5, Zrt-irt-like protein 5; B0AT1, B-0-system neutral amino acid co-transporter; LAT1, L-type amino acid transporter 1; y+LAT2, y+L-type amino acid transporter 2; rBAT, b0,+-type amino acid transporter; EAAT3, excitatory amino acid transporter 3; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; F, forward; R, reverse.