Table 4.
Effect of dietary Zn source on the mRNA expression levels of Zn, amino acid and small peptide transporters in the jejunum of broilers at 28 and 39 d of age.1
| Genes (RQ)2 | D 28 |
D 39 |
||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Control | ZnS | Zn-Prot M | SEM | P-value | Control | ZnS | Zn-Prot M | SEM | P-value | |
| ZnT1 mRNA | 1.04 | 1.04 | 0.85 | 0.05 | 0.253 | 1.02 | 1.04 | 0.94 | 0.04 | 0.617 |
| ZnT4 mRNA | 1.03 | 1.12 | 1.14 | 0.04 | 0.405 | 1.02 | 0.99 | 0.85 | 0.04 | 0.165 |
| ZnT5 mRNA | 1.01 | 1.14 | 1.04 | 0.03 | 0.204 | 1.06 | 0.96 | 0.93 | 0.07 | 0.739 |
| ZnT7 mRNA | 1.01 | 1.12 | 1.05 | 0.04 | 0.552 | 1.04 | 0.96 | 0.99 | 0.05 | 0.822 |
| ZnT9 mRNA | 1.01 | 1.08 | 1.02 | 0.07 | 0.912 | 1.04 | 0.87 | 1.03 | 0.07 | 0.516 |
| ZnT10 mRNA | 1.02b | 1.39a | 1.34a | 0.06 | 0.035 | 1.02 | 0.92 | 0.75 | 0.06 | 0.136 |
| ZIP3 mRNA | 1.03 | 1.02 | 0.89 | 0.05 | 0.477 | 1.02 | 1.00 | 1.14 | 0.04 | 0.366 |
| ZIP5 mRNA | 1.03 | 0.91 | 1.07 | 0.04 | 0.355 | 1.05 | 1.04 | 0.85 | 0.07 | 0.430 |
| B0AT1 mRNA | 1.09 | 1.13 | 1.50 | 0.08 | 0.081 | 1.07 | 1.02 | 1.00 | 0.08 | 0.940 |
| LAT1 mRNA | 1.05 | 1.27 | 1.24 | 0.10 | 0.597 | 1.08 | 1.18 | 0.99 | 0.08 | 0.593 |
| y+LAT2 mRNA | 1.03b | 1.02b | 1.55a | 0.09 | 0.018 | 1.06 | 0.99 | 0.79 | 0.07 | 0.216 |
| rBAT mRNA | 1.02b | 1.26a | 1.23a | 0.04 | 0.022 | 1.01 | 1.11 | 1.30 | 0.11 | 0.559 |
| EAAT3 mRNA | 1.02 | 1.14 | 1.14 | 0.04 | 0.460 | 1.02 | 0.97 | 0.87 | 0.06 | 0.576 |
| PepT1 mRNA | 1.02b | 1.28a | 1.30a | 0.05 | 0.033 | 1.05 | 1.25 | 0.95 | 0.08 | 0.293 |
ZnT1, zinc transporter 1; ZnT4, zinc transporter 4; ZnT5, zinc transporter 5; ZnT7, zinc transporter 7; ZnT9, zinc transporter 9; ZIP3, Zrt-irt-like protein 3; ZIP5, Zrt-irt-like protein 5; B0AT1, B-0-system neutral amino acid co-transporter; LAT1, L-type amino acid transporter 1; y+LAT2, y+ L-type amino acid transporter 2; rBAT, b0,+-type amino acid transporter; EAAT3, excitatory amino acid transporter 3; PepT1, peptide-transporter 1; ZnS, Zn sulfate; Zn-Prot M, Zn proteinate with moderate chelation strength (Qf = 51.6).
,b,cMeans with different superscripts within the same column differ significantly (P < 0.05).
Values are the means of 7/8 replicate cages of 3 birds per replicate cage (n = 7/8).
The mRNA levels were calculated as the relative quantities (RQ) of the target gene mRNA to the geometric mean of β-actin and GAPDH mRNA using the 2−ΔΔCT method.