Table 6.
Genes (RQ)2 | D 28 |
D 39 |
||||||||
---|---|---|---|---|---|---|---|---|---|---|
Control | ZnS | Zn-Prot M | SEM | P-value | Control | ZnS | Zn-Prot M | SEM | P-value | |
ZnT1 | 0.56 | 0.57 | 0.55 | 0.01 | 0.889 | 0.24 | 0.22 | 0.19 | 0.01 | 0.229 |
ZnT4 | 0.29 | 0.28 | 0.28 | 0.01 | 0.907 | 0.42 | 0.37 | 0.34 | 0.02 | 0.279 |
ZnT5 | 0.58 | 0.64 | 0.69 | 0.03 | 0.357 | 0.36 | 0.35 | 0.32 | 0.01 | 0.409 |
ZnT7 | 0.26c | 0.35b | 0.43a | 0.02 | 0.0003 | 0.24 | 0.26 | 0.23 | 0.01 | 0.364 |
ZnT9 | 0.32 | 0.38 | 0.33 | 0.01 | 0.074 | 0.47 | 0.44 | 0.49 | 0.01 | 0.320 |
ZnT10 | 0.36b | 0.31b | 0.48a | 0.03 | 0.022 | 0.37 | 0.28 | 0.34 | 0.02 | 0.189 |
ZIP3 | 0.64 | 0.52 | 0.65 | 0.04 | 0.379 | 0.53b | 0.51b | 0.68a | 0.03 | 0.024 |
ZIP5 | 0.42 | 0.41 | 0.46 | 0.01 | 0.297 | 0.29b | 0.23b | 0.44a | 0.03 | 0.012 |
B0AT1 | 0.31 | 0.47 | 0.43 | 0.06 | 0.581 | 0.58 | 0.34 | 0.38 | 0.05 | 0.080 |
LAT1 | 0.30 | 0.32 | 0.29 | 0.01 | 0.607 | 0.21 | 0.24 | 0.20 | 0.01 | 0.080 |
y+LAT2 | 0.75b | 0.71b | 0.89a | 0.03 | 0.027 | 0.27 | 0.29 | 0.33 | 0.02 | 0.442 |
rBAT | 0.26b | 0.30a | 0.30a | 0.01 | 0.040 | 0.54 | 0.64 | 0.58 | 0.03 | 0.371 |
PepT1 | 0.50 | 0.43 | 0.41 | 0.02 | 0.083 | 0.42 | 0.43 | 0.40 | 0.01 | 0.602 |
ZnT1, zinc transporter 1; ZnT4, zinc transporter 4; ZnT5, zinc transporter 5; ZnT7, zinc transporter 7; ZnT9, zinc transporter 9; ZIP3, zrt-irt-like protein 3; ZIP5, zrt-irt-like protein 5; B0AT1, B-0-system neutral amino acid co-transporter; LAT1, L-type amino acid transporter 1; y+LAT2, y+L-type amino acid transporter 2; rBAT, b0,+-type amino acid transporter; PepT1, peptide-transporter 1; ZnS, Zn sulfate; Zn-Prot M, Zn proteinate with moderate chelation strength (Qf = 51.6).
,b,cMeans with different superscripts within the same column differ significantly (P < 0.05).
Values are the means of 7/8 replicate cages of 3 birds per replicate cage (n = 7/8).
The protein expressions were calculated as the relative quantities (RQ) of the target gene protein to the β-tubulin protein.