FIG. 3.

Conversion of cyclododecanone by E. coli cosmid clones and subclones containing the indicated gene fragments. Region with arrows, the cdd genes identified on the 10,480-bp cluster. Solid horizontal line, flanking sequences of the cdd gene cluster represented on the cosmids or subclones. Vertical line, the start (0) or the end (bp 10480) of the cluster. Other numbers indicate the ends of the fragments relative to the start of the cluster on the cosmids or subclones (positive numbers for downstream, negative numbers for upstream). For product detection, −, absence of metabolite; +, presence of metabolite; +++, 6- to 10-fold more metabolite.