TABLE 2.
Production of 12-hydroxylauric acid and DDDA by E. coli cosmid clonesa
| Cosmid clone | % Conversionb
of:
|
|||
|---|---|---|---|---|
| 12-hydroxylauric acid
from:
|
DDDA from:
|
|||
| CDDAa | CDDK | CDDA | CDDK | |
| Vector control | 0 | 0 | 0 | 0 |
| S8C | 0.27 | 56.1 | 0 | 1.7 |
| D12D | 0.62 | 24.1 | 0 | 0.04 |
| O8E | 0.20 | 4.1 | 0 | 0.021 |
a
Assay was performed in M9 plus glucose medium. Cyclododecanol (CDDA) or cyclododecanone (CDDK) (7.5 mg of either) was added as the substrate for biotransformation.
b
Products were quantitated by GC analysis. Percent conversion was calculated as the percentage of 12-hydroxylauric acid or DDDA produced from either the CDDA or CDDK substrate.