TABLE 1.
Strain | Relevant genotype or (phenotype) | % cI+a
|
|
---|---|---|---|
No plasmid | With pMutS+ | ||
CC106 | mutS+mutL+vsr+ | 3.2 | 2.3 |
GM30 | mutS+mutL+vsr+ | 3.1b | 1.6b |
GM31 | GM30 dcm-6 (Vsr−) | 0.49b | 0.18b |
SP1 | mutS::Tn10 mutL+vsr+ | 0.83 | 1.1 |
KMBL3752 | mutS+mutL+vsr+ | 5.0 | 3.9 |
KMBL3760 | mutS+mutL-101 vsr+ | 0.64 | 0.34 |
The values are percentages of cI+ among N+O+ recombinants in a cross of λNcIam6 O+ with λN+ cICP7 O and are averages of results from two or more experiments. During recombination, heteroduplexes containing T · G mismatches are generated which are repaired by VSP repair to create cI+ phage. Thus, a higher percentage of cI+ indicates more VSP repair (see references 12 and 15). Plasmid pMutS+ has been described previously (21).
Value from reference 15.