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. 2001 Nov;183(21):6487–6490. doi: 10.1128/JB.183.21.6487-6490.2001

TABLE 1.

Effects of excess MutS+ on VSP repair

Strain Relevant genotype or (phenotype) % cI+a
No plasmid With pMutS+
CC106 mutS+mutL+vsr+ 3.2 2.3
GM30 mutS+mutL+vsr+ 3.1b 1.6b
GM31 GM30 dcm-6 (Vsr) 0.49b 0.18b
SP1 mutS::Tn10 mutL+vsr+ 0.83 1.1
KMBL3752 mutS+mutL+vsr+ 5.0 3.9
KMBL3760 mutS+mutL-101 vsr+ 0.64 0.34
a

The values are percentages of cI+ among N+O+ recombinants in a cross of λNcIam6 O+ with λN+ cICP7 O and are averages of results from two or more experiments. During recombination, heteroduplexes containing T · G mismatches are generated which are repaired by VSP repair to create cI+ phage. Thus, a higher percentage of cI+ indicates more VSP repair (see references 12 and 15). Plasmid pMutS+ has been described previously (21). 

b

Value from reference 15