TABLE 2.

VSP repair in bacteria containing mutS plasmids

mutS plasmid		VSP repair in bacteria with indicated genotype				Complementing protein(s)	Class
		mutS+		mutS mutant
USCe no.	aa change	% cI+a	Relative value	% cI+	Relative value
	Nonef	3.17b	1.00	0.83b	1.00
1	None (WT)d	2.26	0.71	1.07b	1.29
24	G117D	1.16	0.37	NDc			II
2	G117S	3.31	1.04	1.29	1.57	MutS	I
22	A123V	1.77	0.55	ND	ND		II
8	R305H	4.02	1.27	0.97b	1.18	MutS	I
23	R350H	1.62	0.51	ND			II
11	G583D	0.42	0.13	0.23	0.28		II
3	G614D	1.12	0.35	0.25	0.30		II
5	G618S	0.52	0.16	0.23b	0.27	MutS	II
9	G619D	0.16	0.05	0.14	0.17		II
6	S621N	3.23	1.02	4.51	5.50	MutS MutH	I
7	D659N	ND		0.80	0.98	MutS MutH	I
10	S668L	0.18	0.06	0.19b	0.23		II
14	T669I	3.04b	1.24	4.06	4.95	MutS MutL MutH	I
4	E673K	3.01	0.97	1.52b	1.85	MutS MutH	I
12	D693N	2.89	0.89	2.08	2.50	MutS MutH	I
21	E694K	4.16	1.31	ND		MutS	I
16	G698E	3.20	1.01	2.14	2.58	MutS MutL MutH	I

^a% cI⁺, the number of cI⁺ phage/total number of recombinant phages. 

^bAverage of values from two or more crosses. The mutS⁺ strain was CC106, and the mutS strain was SP1. 

^cND, not determined. 

^dPlasmid with wild-type (WT) mutS. 

^eUSC, University of Southern California collection. 

^fNo plasmid.