Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Mar 13;25(3):381–389. doi: 10.1038/s41556-023-01095-y

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 2 — a, Relative expression levels of viral RNA in WT and CIART^−/− hPSC-CMs at 24 h.p.i. with SARS-CoV-2 (m.o.i. = 0.3). b,c, Representative confocal images (b) and calculated percentages (c) of SARS-N⁺ cells within the cTnT⁺ cell populations of WT and CIART^−/− hPSC-CMs at 24 h.p.i. with SARS-CoV-2 (m.o.i. = 0.3). d, Relative expression levels of viral RNA in WT and CIART^−/− hPSC-AWOs at 24 h.p.i. with SARS-CoV-2 (m.o.i. = 0.3). e,f, Representative confocal images (e) and calculated percentages (f) of SARS-N⁺ cells within the FOXJ1⁺ cell populations of WT and CIART^−/− hPSC-AWOs at 24 h.p.i. with SARS-CoV-2 (m.o.i. = 0.3). g, Relative expression levels of viral RNA in WT and CIART^−/− hPSC-ALOs at 24 h.p.i. with SARS-CoV-2 (m.o.i. = 0.3). h,i, Representative confocal images (h) and calculated percentages (i) of SARS-N⁺ cells in the mature SP-B⁺ cell populations of WT and CIART^−/− hPSC-ALOs at 24 h.p.i. with SARS-CoV-2 (m.o.i. = 0.3). j,k, Representative confocal images (j) and calculated percentages (k) of SARS-N⁺ cells in the mature SP-C⁺ cell populations of WT and CIART^−/− hPSC-ALOs at 24 h.p.i. with SARS-CoV-2 (m.o.i. = 0.3). a–k, n = 3 independent biological replicates. l, Percentage of viral UMI counts in mock- and SARS-CoV-2-infected WT and CIART^−/− hPSC-AWOs (m.o.i. = 0.1; 24 h.p.i.). m, Uniform manifold approximation and projection (UMAP) plot illustrating five cell clusters in the hPSC-AWOs. n, Correlation analysis of cell clusters in hPSC-AWOs and adult human lung. o, Percentage of viral UMI counts in each cluster of WT and CIART^−/− hPSC-AWOs following SARS-CoV-2 infection (m.o.i. = 0.1; 24 h.p.i.). p, Levels of SARS-CoV-2 viral transcripts in ciliated-like cells 1 of WT and CIART^−/− hPSC-AWOs following SARS-CoV-2 infection (m.o.i. = 0.1; 24 h.p.i.). Data are the mean ± s.d. P values were calculated using an unpaired two-tailed Student’s t-test. Scale bars, 100 μm.

Source data