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. 2023 Jan 23;25(3):467–480. doi: 10.1038/s41556-022-01074-9

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 8 — a, Immunostaining (TOM20) of mitochondria in sHSP-depleted (depl), knockout (KO), or knockdown (KD) cell lines. The HeLa cell lines with reduced levels of all sHSPs and severe mitochondrial defects (used in Fig. 3) were generated by the combination of CRISPR/Cas9 knockouts and short hairpin RNAs (shRNA). Knockout or knockdown of individual components (HSPB1, HSPB5, HSPB8) did not present alterations in mitochondrial morphology compared to wild-type HeLa cells. Scale bars represent 10 μm and 2.5 μm (Zoom). b,c, Immunostaining of endoplasmic reticulum (KDEL) (b) and Golgi apparatus (Giantin) (c) in control (WT) and sHSP-depleted (depl) cell lines. Scale bars represent 10 μm. Images are representative of two (b,c) or three (a) replicates.