FIG. 4.

Growth of E. coli expressing L. interrogans ChpK in the presence or absence of 100 μM IPTG. Cells harboring pET30a, pTAK (chpK), and pTAKI (chpK chpL) are indicated. Plates supplemented with 50 μg of kanamycin (Km) per ml were incubated for 24 h at 37°C.