Immunofluorescence of Escherichia coli (ATCC 35150 GFP) expressing GFP adhering to a basolateral-out enteroid, whole mount image taken at 20X, 100μM scale bar (A). Percent of treated E. coli adhering to basolateral-out NEC derived vs non-NEC derived enteroids, n=3 in each group, p=0.0329 (B). Whole mount image of E.coli adhering to apical-out enteroids, taken at 20X, 100μM scale bar (C). Percent of treated E. coli adhering to apical-out NEC derived vs non-NEC derived enteroids, n=3, p>0.05 (D).