Fig. 4. Extension of Tz to other fluorophores to tune probe emission spectra.

A Structures of the Tz-tethered probes. B Fluorescence spectra of the probes before or after the treatment of various analytes. C Photophysical properties of the probes before or after the treatment of superoxide. ^[a]Ф: Quantum yields of F-Tz5/6 were determined at an excitation wavelength of 365 nm and using quinine sulfate (Ф_standard = 0.577 in 0.1 M H₂SO₄) as a standard. Quantum yields of F-Tz7 were determined at an excitation wavelength of 460 nm and using fluorescein (Ф_standard = 0.95 in 0.1 M NaOH) as a standard. Quantum yields of F-Tz8/9 were determined at an excitation wavelength of 470 nm and using fluorescein (Ф_standard = 0.95 in 0.1 M NaOH) as a standard. D Imaging superoxide in live cells with these probes. Scale bar: 25 μm. Representative images are shown from n = 3 independent experiments.